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OBJECTI VE To establish the high performan ce liquid c hromatography（HPLC）fingerprint of carotenoid in Lycium barbarum，and to investigate the spectrum-effect relationship between its common peak and antioxidant activity. METHODS HPLC method was adopted. The fingerprints of carotenoid in 34 batches of L. barbarum from different producing areas were established by Similarity Evaluation System of TCM Fingerprint （2012 edition），and similarity evaluation and common peak identification were carried out. Taking scavenging rate of DPPH free radical as index ，in vitro antioxidant activity of carotenoid in L. barbarum was investigated. The spectrum-effect relationship between the common peaks of carotenoids in L. barbarum and antioxidant activity was analyzed by grey correlation method. RESULTS There were 4 common peaks in the fingerprints of carotenoids in 34 batches of L. barbarum ，and the similarity was not less than 0.903. Peak 1 was identified as zeaxanthin ，and peak 4 as zeaxanthin dipalmitate. The scavenging rates of them to DPPH free radical were 1.792％-3.160％. The common peaks of carotenoids in L. barbarum were positively correlated with scavenging rate of DPPH free radical ，and the correlation degree was greater than 0.6；the correlation degree of peak 2 and peak 4（zeaxanthin dipalmitate ）with scavenging rate of DPPH free radical was greater than 0.8. According to the correlation degree ，the contribution of each common peak to scavenging rate of DPPH free radical was determined as peak 2＞ peak 4（zeaxanthin dipalmitate ）＞peak 1（zeaxanthin）＞peak 3. CONCLUSIONS In this study ，HPLC fingerprint of carotenoid in L. barbarum is successfully established ，and two common peaks are identified. The chemical components represented by peak 2 and zeaxanthin palmitate may be the material basis of antioxidant activity of carotenoid in L. barbarum .
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Objective:To analyze original medical humanities articles written by staff of the hospital and extract key elements of hospital culture for staff perception, for recommendations on homogenous development of multiple campuses of the hospital.Methods:Original medical humanities articles written by staff of the hospital were collected from the WeChat accounts, websites and printouts of a tertiary hospital in Beijing from January 2013 to December 2019, with text mining made by Python3.7; Bert and Tencent open-source 8 million Chinese word dataset were used as entity identification training dataset; K-Means cluster analysis was used to analyze and select cluster results of higher degree of fitting; TextRank was used to screen keywords of each clustered category. Significance of each category was summarized in the end based on keywords following the screening.Results:Among the 341 articles collected, high frequency words were work, hospital, patient, sick person, development, medicine, medical treatment, doctor, outpatient service and clinic. The words fell into ten categories: process recall, work attribute, work responsibility, diagnosis and treatment behavior, growth experience, team cooperation, professionalism, management innovation, doctor-patient communication and others.Conclusions:Research on hospital staff culture perception is conducive to identifying the cultural content of the hospital. In the homogenous development of multiple campuses of the hospital, it is imperative to nurture the staff′s sense of identify and belonging to the hospital, enhance their sense of gain and happiness, and strengthen the scientific management system centering on patients.
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Peking University Third Hospital was cited as an example in this paper to study how to guide public opinion and build a harmonious doctor-patient relationship by using WeChat official platform.It pointed out that the public relations office of the hospital should keep track of the ongoing healthcare reform,and leverage the functions of mass media,improve professionalism of their media workers,and build a long-term mechanism to response to public opinions in time.
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<p><b>OBJECTIVE</b>To detect the DNA damage of the nurses occupationally exposed to anticancer drugs and to assess the exposure level using Comet assay.</p><p><b>METHODS</b>Sixteen nurses occupationally exposed to anticancer drugs were selected as exposure group, the average exposure period was 5.6 years, and the average exposure dose was to prepare 7.8 portions of anticancer drugs daily. Meanwhile, sixteen nurse students were selected as control group. The DNA migration of the peripheral lymphocytes of both groups was detected using comet assay.</p><p><b>RESULTS</b>The comet length was 46.27 microns in exposure group, which was significantly higher than that of control group (26.78 microns, P < 0.01). Also the percentage of long tailed nucleus (LTN) of exposure group was 64.83%, which was significantly higher than that of control group (4.87%, P < 0.01).</p><p><b>CONCLUSIONS</b>There was DNA damage in the nurses occupationally exposed to antineoplastic drugs.</p>